Properties. Clones of transfected cells were selected in the presence of geneticin (also known as G418). We used the cells to inoculate a T-175 flask containing 20 mL of pre-warmed medium. This experiment was performed twice, and one representative graph is shown. ( B ) EBOV titers are similar in ATCC Vero E6 cells plated 24, 48 or 72 hours prior to plaque assay. The cells are amplified through subcultures in TC-flasks using animal origin-free trypsin-like enzyme, and SFM. The * indicates p = 0.006 between 24 and 72 hour samples for passage 29. Vero/hSLAM was derived by transfection of Vero cells with a vector plasmid (pCXN2) containing the neomycin resistance gene and an expression plasmid (pCAG-hSLAM) encoding the human signaling lymphocytic activation molecule (SLAM) , also known as CDw150, which is a receptor for measles virus. *Suppliers of these products at the time of this study. Related Categories: CITES, Cell Culture, ECACC Cell Lines, S-Z: biological … ATCC vero cells Vero Cells, supplied by ATCC, used in various techniques. Vero… The cell suspension is distributed in cryogenic vials with 6–8×10 High-Density Vero Cell Perfusion Culture in BioBLU ... (5 x 106 cells) following the Vero cell protocol provided by ATCC [4].
Bioz Stars score: 94/100, based on 732 PubMed citations. We cultured the cells in a Galaxy ® 170R CO 2 incubator (Eppendorf, Germany) at 37°C and 5 % CO 2, with passive humidification. Vero cells can be purchased from ATCC and the European Collection of Animal Cell. Each bar represents an average of 5 replicates. Vero cells are sensitive to infection with SV-40, SV-5, measles, arboviruses, reoviruses, rubella, simian adenoviruses, polioviruses, influenza viruses, parainfluenza viruses, respiratory syncytial viruses, vaccinia, and others. ZERO BIAS - scores, article reviews, protocol conditions and more Vero cells from these flasks were used to prepare the Master Cell Bank (MCB). Vero cells (ATCC CCL81.4) are maintained for four passages in SFM for cell adaptation to this medium. VERO Cell Line from African green monkey kidney, 84113001, A fibroblast-like kidney cell, non-WHO origin, Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES) Purchase; Safety & Documentation; Protocols & Articles; Peer-Reviewed Papers; Related Products; Purchase .
Materials used in this study are described below. Methods Bioreactor setup and microcarrier preparation The S.U.B. Cultures (ECACC) repositories. Vero cells (ATCC, CCL-81 ™ cells) were adapted to HyQ SFM4MegaVir™ medium, which was developed to support virus production in a variety of cell lines. ( A ) Vero and Vero E6 cells produce EBOV plaques. Commonly used Vero cell lines from the ATCC include.
Description: Vero/hSLAM was derived by transfection of Vero cells with a vector plasmid (pCXN2) containing the neomycin resistance gene and an expression plasmid (pCAG-hSLAM) encoding the human signaling lymphocytic activation molecule (SLAM) , also known as CDw150, which is a receptor for measles virus.Clones of transfected cells were selected in the presence of geneticin (also known …
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